Method for rapidly screening low-temperature and weak-light breeding material of cucumis melo L.var.conomon Group

The invention discloses a method for rapidly screening a low-temperature and weak-light breeding material of cucumis melo L.var.conomon Group, and relates to the technical field of genetic breeding. Firstly, embryo fetching conditions of the crisp melons are determined, wherein 16-18 days after flowering and pollination, peeled seeds obtained under the conditions have a high survival rate after being subjected to tissue culture and germinate 10 days earlier than mature seeds; secondly, the best culture medium for the peeled seeds is determined, wherein the culture medium contains 950 mg/L potassium nitrate, 825 mg/L ammonium nitrate, 85 mg/L potassium dihydrogen phosphate, 90 mg/L anhydrous magnesium sulfate, 165 mg/L anhydrous calcium chloride, 0.83 mg/L potassium iodide, 6.2 mg/L boric acid, 16.9 mg/L manganese sulfate monohydrate, 8.6 mg/L zinc vitriol, 0.25 mg/L sodium molybdate dihydrate, 0.025 mg/L copper sulfate pentahydrate, 0.025 mg/L cobalt chloride hexahydrate, 37.26 mg/L ethylene diamine tetraacetic acid disodium, 15.2 mg/L anhydrous ferrous sulfate, 8,500 mg/L agar powder, 18,000 mg/L sucrose and the like; meanwhile, the best conditions for adversity identification when obtained seedlings of cucumis melo L.var.conomon Group are cultured to the stage of cotyledon unfolding are determined, wherein the daytime temperature is 15-18 DEG C, and the night temperature is 12-15 DEG C. By means of the method, the target material can be rapidly screened.