High-efficiency, rapid and low-cost method of obtaining cassava tissue culture seedlings

[0014] Embodiment 1: the preparation of cassava tissue culture seedling

[0015] 1. Preparation of semi-solid medium:

[0016] The semi-solid medium was prepared according to the formula in Table 1, with a pH value of 5.8, sterilized under high temperature and high pressure at 115°C for 30 minutes, and solidified for later use.

[0017] Table 1 Medium composition and dosage

[0018]

[0019] 2. Surface disinfection of cassava explants:

[0020] The surface disinfection of cassava explants was mainly carried out using 75% alcohol and sodium hypochlorite solution. Take the young twigs with bud eyes of Huanan No. 124 cassava, cut off the leaves, and keep the petiole about 3cm long, wash it with detergent water and then rinse it with running water for 20 minutes, put it in an ultra-clean workbench, and rinse it with 75% alcohol for 10 seconds , washed twice with sterile water, and then soaked in 1% sodium hypochlorite solution by volume for 10 minutes, shaking from time to time