Tissue culture method for increasing banana survival rate

[0017] A kind of tissue culture method improving banana survival rate of the present invention, the steps are as follows:

[0018] (1) Induction culture: select banana suction buds as explants, sterilize the explants, and transfer them to induction medium for cultivation. The formula of the induction medium is: MS medium + 6-BA 12mg / L+pear juice 1.1g / L+NAA 0.55g / L+sucrose 18g / L+agar 6g / L; induction culture conditions: dark culture, temperature 29°C;

[0019] (2) Subculture: transfer the clustered buds after induction into the subculture medium for cultivation; the formula of the subculture medium is: MS medium + 0.06g / L+naphthaleneacetic acid 0.1g / L+apple juice 0.02g / L+sucrose 18g / L+agar 6g / L; the subculture conditions are: sunlight for 16 hours a day, light intensity 900Lux, temperature 29°C;

[0020] (3) Rooting culture: transfer the subcultured banana seedlings to the rooting medium for cultivation; the formula of the rooting medium is: MS medium + 6-BA

[0023] A kind of tissue culture method improving banana survival rate of the present invention, the steps are as follows:

[0024] (1) Induction culture: select banana suckers as explants, sterilize the explants, and transfer them to induction medium for cultivation. The formula of the induction medium is: MS medium + 6-BA 10mg / L+pear juice 1.2g / L+NAA 0.6g / L+sucrose 15g / L+agar 6g / L; induction culture conditions: dark culture, temperature 30°C;

[0025] (2) Subculture: transfer the clustered shoots after induction into the subculture medium for cultivation; the formula of the subculture medium is: MS medium + 2,4-dichlorophenoxyacetic acid 0.08g / L+naphthaleneacetic acid 0.08g / L+apple juice 0.03g / L+sucrose 20g / L+agar 6g / L; the subculture conditions are: sunlight for 15 hours a day, light intensity 1000Lux, temperature 30°C;

[0026] (3) Rooting culture: transfer the subcultured banana seedlings to the rooting medium for cultivation; the formula of the rooting

[0029] A kind of tissue culture method improving banana survival rate of the present invention, the steps are as follows:

[0030] (1) Induction culture: select banana suckers as explants, sterilize the explants, and transfer them to induction medium for cultivation. The formula of the induction medium is: MS medium + 6-BA 15mg / L+pear juice 1.0g / L+NAA 0.5g / L+sucrose 20g / L+agar 6g / L; induction culture conditions: dark culture, temperature 28°C;

[0031] (2) Subculture: transfer the clustered shoots after induction into the subculture medium for cultivation; the formula of the subculture medium is: MS medium + 0.05g / L+naphthaleneacetic acid 0.12g / L+apple juice 0.01g / L+sucrose 15g / L+agar 6g / L; the subculture conditions are: sunlight for 18 hours a day, light intensity 800Lux, temperature 28°C;

[0032] (3) Rooting culture: transfer the subcultured banana seedlings to the rooting medium for cultivation; the formula of the rooting medium is: MS medium + 6-BA 0.5