Novel material and method for relieving heavy metal stress of crambe

[0024] A novel material and method for alleviating heavy metal stress in sea kale, comprising the following steps:

[0025] (1) Preparation of SNPs suspension

[0026] Weigh the SNPs in a sterile environment and pour them into a pre-sterilized triangular flask filled with deionized water to prepare a SNPs mother solution with a concentration of 1g / L. Under the condition of 30-45kHz, ultrasonically disperse for 40-60min to obtain the SNPs suspension, and put it on the ultra-clean workbench for later use;

[0027] (2) Preparation of As(Ⅴ) solution

[0028] Weigh Na in a sterile environment 2 HASO 4 ·7H 2 O was poured into a pre-sterilized centrifuge tube filled with deionized water and mixed evenly, and an As(Ⅴ) solution with a concentration of 1000 μM was prepared and placed in an ultra-clean workbench for standby;

[0029] (3) Preparation of 1 / 2MS medium containing As(Ⅴ) and SNPs

[0030] After the 1 / 2MS medium is sterilized, place it in an ultra-clean workbench to co

[0036] A kind of novel material and method for mitigating the heavy metal stress of sea cabbage, different from embodiment 1, comprises the following steps:

[0037] (1) Step (3) in the preparation of the 1 / 2MS medium containing As(Ⅴ) and SNPs:

[0038] Add a certain volume of prepared SNPs suspension, sterilized water and As(Ⅴ) solution to the culture medium quickly before the culture medium solidifies to ensure that the concentration of SNPs in the culture medium is 100 mg / L, and the concentration of As(Ⅴ) is 350 μM.

[0039] (2) In the cultivation of step (5) sea cabbage:

[0040] Move the inoculated culture box to an incubator at a temperature of 20°C, a daily light and dark time of 15h / 9h, and a light intensity of 230μM m -2 ·s -1 cultivated under conditions.

[0042] A kind of novel material and method for mitigating the heavy metal stress of sea cabbage, different from embodiment 1, comprises the following steps:

[0043] (1) Step (3) in the preparation of the 1 / 2MS medium containing As(Ⅴ) and SNPs:

[0044] Before the medium solidifies, quickly add a certain volume of prepared SNPs suspension, As(Ⅴ) solution, and sterilized water into the medium to ensure that the concentration of SNPs in the medium is 200 mg / L, and the concentration of As(Ⅴ) is 250 μM.

[0045] (2) In the cultivation of step (5) sea cabbage:

[0046] Move the inoculated culture box to the incubator at a temperature of 21°C, a daily light and dark time of 17h / 7h, and a light intensity of 270μM m -2 ·s -1 cultivated under conditions.

[0047] The following is Hg 2+ Examples of the effects of different concentrations of SNPs on the growth of sea cabbage under stress.