The invention provides a
tissue culture and rapid propagation method for firmiana major, and belongs to the technical field of
plant tissue culture. The method is characterized in that tender terminalbuds or lateral buds of firmiana major are used as explants; through explant disinfection and a series of culture including induction, differentiation, proliferation and rooting in sequence on a specific culture medium, the problems of
tissue culture, rapid propagation, introduction and
domestication, protection, and development and utilization of scientific research and
landscaping of firmiana major are effectively solved; and meanwhile, natural
plant quantity reduction and natural
vegetation area damage caused by natural disappearance, death and excessive utilization of wild resources are effectively avoided, and particularly, the method plays a positive role in maintaining the stability of special characters. According to the method, the induced differentiation rate is 78%, the propagation period is 60 days, the propagation coefficient is 3, the rooting rate is 91%, the
transplanting survival rate is 95% or above, the propagation number and growth rate of firmiana major are greatlyincreased, and a
technical support is provided for protection and propagation, introduction and
domestication, preservation and large-scale production of the species.