Corn bud with high activity of saponin glycosidase and use thereof

[0011] Wash 1 kg of barley with water, soak in germination water at 16°C for 4 to 8 hours, and after germination for 12 to 16 hours, use 50 ml of commercially available gibberellic acid and 15 mg of ginsenosides in an aqueous solution or buffer solution (acetic acid buffer, phosphate buffer or tris buffer) sprayed and mixed, germinated for 4 to 5 days at 16°C, and dried (drying method, edited by Guan Dunyi: Handbook of Beer Industry, China Light Industry Press, 1998; p99- 120), that is, to make malt with high ginsenoside glucosidase activity.

[0012] 500 grams of the above-mentioned malts (calculated by dry weight) were mashed, added to 3000 milliliters of 0.02 mole, pH 5.0 acetate buffer and soaked for one hour, centrifuged to remove impurities, the supernatant was added ammonium sulfate with a saturation of 66%, and the precipitate was collected by centrifugation. Dialyze with 0.02 molar, pH 5.0 acetic acid buffer solution to remove salt, then use the buffer solution to fix th

[0018] Wash 1 kg of barley with water, soak in germination water at 16°C for 4 to 8 hours, and after germination for 12 to 16 hours, use 50 ml of gibberellic acid and 8 mg of Pulsatilla saponin (according to the literature: "Liu Lin etc., Journal of Dalian Institute of Light Industry, 23 (1), 18-21, 2000 "method extraction) aqueous solution or buffer solution (acetate buffer, phosphate buffer or tris buffer) solution spray mixing, 16 ℃ to continue to germinate 4 ~ After 5 days, dry (drying method, edited by Guan Dunyi: Handbook of Beer Industry, China Light Industry Press, 1998; p99-120), that is, malt with high Pulsatilla saponin glycosidase activity is made.

[0019] Extract enzyme and process Pulsatilla saponin according to the method of Example 1; Its reactant is measured with thin-layer chromatography (TLC) and high-performance liquid chromatography (Wang Wei: Journal of Dalian Institute of Light Industry, 24 (1), 4-7, 2005) Results: 80% of Pulsatilla saponins with 5 sugar g

[0022] Wash 1 kg of barley with water, immerse in germination water at 16°C for 4 to 8 hours, and then germinate for 12 to 16 hours. solution, phosphate buffer or tris buffer), spray and mix, continue to germinate at 16°C for 4 to 5 days, and dry (drying method, edited by Guan Dunyi: Handbook of Beer Industry, China Light Industry Press, 1998; p99-120), ready-made into malt with high rutinase activity.

[0023] Extract enzyme and process rutin according to embodiment 1,2 method; Its reactant uses thin-layer chromatography (TLC) and high-performance liquid chromatography (Wei Shenghua: Journal of Dalian Institute of Light Industry, 22 (2), 114-116, 2003) Determination results: 85% of rutin with 2 sugar groups was degraded into isoquercetin and quercetin with 1 sugar group.

[0024] The rutin added in the above malt preparation process is replaced by Epimedium or soybean isoflavone glycosides (glycosides) to prepare malt, and the prepared malt is used to enzymatically hydrolyze Ep