Lithocarpus carolinae tissue culture explant obtaining method

[0025] Select the fine family seeds of Hongxinke with no pests and diseases and full grains. After routine disinfection and hot water soaking, the seeds are dried in an oven at 100-140°C. After natural cooling to room temperature, the seeds are sown in a 0.3% volume concentration. Sand bed disinfected with potassium permanganate solution.

[0026] After the sprouts are unearthed, when the sprouts are 6-8 cm high, they are transplanted into a non-woven seedling bag with a size of 15cm×15cm, sprayed with water and sprayed according to the situation, and apply a compound with a mass concentration of 0.5% every 7-10 days. Fertilizer solution, wherein the active ingredient of NPK in the compound fertilizer solution is more than or equal to 48%, and the sprouts will take root after 40 days.

[0027] When the transplanted seedlings take root and stabilize, select container seedlings with a seedling height of 40-60 cm, a ground diameter of 0.3-0.6 cm, a straight trunk, and few side branc

[0030] Select the seeds of the excellent family of Hongxinke with no pests and diseases and full grains. After routine disinfection and hot water soaking, the seeds are dried in an oven at 110°C. Potassium acid solution disinfected sand bed.

[0031] After the sprouts are unearthed, when the sprouts are 6-8 cm high, they are transplanted into a non-woven seedling bag with a size of 15cm×15cm, watered and sprayed according to the situation, and a 0.5% compound fertilizer solution with a mass concentration of 0.5% is applied every 8 days , wherein the active ingredient of NPK in the compound fertilizer solution is ≥48%, and the sprouts will take root after 40 days.

[0032] After the transplanted seedlings take root and stabilize, select container seedlings with a seedling height of 45-50 cm, a ground diameter of 0.3-0.4 cm, a straight trunk, and few side branches as the mother plant, and transplant them into a container cup with a size of 30 cm × 30 cm and a substrate. In, the co

[0035] Select the fine family seeds of Hongxinke with no pests and diseases and full grains. After routine disinfection and hot water soaking, the seeds are dried in an oven at 120°C. After natural cooling to room temperature, the seeds are sown in high manganese with a volume concentration of 0.3%. Potassium acid solution disinfected sand bed.

[0036] After the sprouts are unearthed, when the sprouts are 6-8 cm high, they are transplanted into a non-woven seedling bag with a size of 15cm×15cm, watered and sprayed according to the situation, and a 0.5% compound fertilizer solution with a mass concentration of 0.5% is applied every 9 days , wherein the active ingredient of NPK in the compound fertilizer solution is ≥48%, and the sprouts will take root after 40 days.

[0037]After the transplanted seedlings take root and stabilize, select container seedlings with a seedling height of 50-55 cm, a ground diameter of 0.4-0.5 cm, a straight trunk, and few side branches as the mother p