3 results about "Hydrolase" patented technology

The invention discloses functional mulberry leaf oligosaccharide and a preparation method and application thereof. The method comprises the following steps that 1, dried mulberry leaves serve as raw materials, after crushing is conducted, degreasing is conducted by means of petroleum ether, and degreased mulberry leaf powder is obtained; 2, a mulberry leaf polysaccharide extracting solution is obtained by conducting water extraction on the degreased mulberry leaf powder; 3, hydrolase is added in the obtained mulberry leaf polysaccharide extracting solution, and oligosaccharide hydrolysate is obtained through enzymolysis; 4, ethanol is added in the oligosaccharide hydrolysate, unreacted mulberry leaf polysaccharide is subjected to alcohol precipitation, and a mulberry leaf oligosaccharide solution with polysaccharide removed is obtained; 5, saccharomyces cerevisiae is added in the mulberry leaf oligosaccharide solution with the polysaccharide removed, fermentation is conducted to removemonosaccharide, and finally the functional mulberry leaf oligosaccharide is obtained. Accordingly, alcohol precipitation is utilized for removing the polysaccharide, microbial fermentation is utilized for removing the monosaccharide, the mulberry leaf oligosaccharide separation and purification process is simplified, the functional mulberry leaf oligosaccharide has better economical efficiency and environmental friendliness, and the obtained mulberry leaf oligosaccharide has the physiological efficacy of promoting proliferation of intestinal beneficial bacteria and inhibiting growth of harmful bacteria.