Solid microbial agent to remedy soil contaminated by petroleum, preparation method thereof and application

[0027] Embodiment 1: the preparation of oil-contaminated soil remediation bacterial agent

[0028] The bacteria used are the aforementioned Acinetobacter radiata and Rhodococcus erythropolis, which are cultured on a slant, prepared seed liquid, liquid-state fermentation culture, and solid-state fermentation culture to obtain the repair bacteria agent. The specific operation steps are:

[0029] (1) Slant culture: Prepare beef extract-peptone slant medium by conventional methods. The specific steps are: 3g beef extract, 5g peptone, 5g NaCl, 1000mL distilled water, pH7.2-7.4, 1.5%-2% agar, extinguished at 121°C Bacteria 20 ~ 30min. Make a slant after sterilization, transfer the preserved strain to the test tube slant, and cultivate it at 25-30°C for 16 hours before use;

[0030](2) Preparation of primary seed liquid: the liquid medium is still beef extract peptone medium, and the prepared liquid medium is divided into 150 or 250 mL Erlenmeyer flasks with a liquid volume of 50 mL, a

[0034] Embodiment 2: the preparation of oil-contaminated soil remediation bacterial agent

[0035] The materials and parameters used in Example 1 are basically the same, except that in the liquid fermentation culture: the inoculation amount of the primary seed liquid is 10% of the volume of the liquid fermentation medium, and after inoculation, it is placed on a shaking table and cultivated for 32h at a temperature of 30°C. ℃, the shaker speed is 250rpm, the effective number of viable bacteria of Acinetobacter radioresistant and Rhodococcus erythropolis is about 5.0×10 8 CFU / mL and 2.5×10 8 CFU / mL; in solid-state fermentation culture: the culture medium is a mixture of high-temperature sterilized bran and peat with a weight ratio of 2:1, the water content of the medium is adjusted to 60%, the inoculation amount of the fermentation broth is 10%, and the culture time is 4 day, in the finished product of the solid microbial agent for remediation of oil-contaminated soil, the ef

[0036] Embodiment 3: the preparation of oil-contaminated soil remediation bacterial agent

[0037] The materials and parameters used in Example 1 or 2 are basically the same, but the difference is that in the liquid fermentation culture: the inoculation amount of the primary seed liquid is 7% of the volume of the liquid fermentation medium, and after inoculation, it is placed on a shaking table and cultivated for 30h. at 28°C, with a shaker rotation speed of 200rpm, the effective viable counts of Acinetobacter radioresistant and Rhodococcus erythropolis were about 4.5×10 8 CFU / mL and 2.0×10 8 CFU / mL; in solid-state fermentation culture: the culture medium is a mixture of bran and peat with a weight ratio of 1:1 after high-temperature sterilization, the water content of the medium is adjusted to 45%, the inoculation amount of the fermentation broth is 8%, and the culture time is 3 day, in the finished product of the solid microbial agent for remediation of oil-contaminated so