1 results about "Porcine epidemic diarrhea virus" patented technology

The invention relates to the field of inspection and quarantine and aims to provide a dual fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection kit for detectingPEDV-TGEV (Porcine Epidemic Diarrhea Virus-Transmissible Gastroenteritis Virus) nucleic acid. The kit comprises (1) an inverse transcription and fluorescent quantitative RT-PCR amplification mixed liquid, wherein the mixed liquid comprises an inverse transcription and fluorescent quantitative amplification primer and a probe and has sequence of SEQ ID NO: 1-6 as shown in the specification, and the inverse transcription primer is a 12 basic group random primer; (2) negative control; (3) positive control, wherein the positive control comprises positive control plasmid for the porcine epidemic diarrhea virus and the transmissible gastroenteritis virus. Compared with the prior art, the kit has the advantages of being high in flux, low in cost, good in specificity and high in sensitivity.