4 results about "Chain reaction" patented technology

The invention relates to the technical field of biosensor, in particular to a fluorescence biosensor based on amplification of a hybridization chain reaction. The problems that the specificity and sensitivity of the method for detecting the kanamycin is low, and the cost is high in the above prior art are solved. According to the fluorescence biosensor based on the amplification of the hybridization chain reaction, the cyclic amplification effect is achieved through the hybridization chain reaction between the endonuclease IV and the chain. The mixture is reacted homogeneously by fluorescenceresonance energy transfer of the fluorophores and the quenching group. The preparation method comprises the following steps of: preparing gold nanoparticles; modifying Walker and Track to the surfaceof gold nanoparticles; mixing the labeled gold nano solution with the homogeneous reaction solution; performing hybridization chain reaction, and detecting the fluorescence; using the specific recognition of the nucleic acid Aptamer, using the nucleic acid Aptamer for high specific detection of the target kanamycin; and using the amplification of the hybridization chain reaction to achieve signalamplification.

The invention relates to a fluorescence analysis method based on HCR and cation exchange reaction of hydrogel, and the method comprises the following steps: modifying a C-C double bond on the surfaceof a substrate through a silanization reaction, and constructing a PEG hydrogel array on the substrate by means of a photomask through a free radical polymerization reaction initiated by ultraviolet light; opening a hairpin structure of a capture probe modified on the hydrogel by the target molecule, initiating a hybridization chain reaction between biotinylation hairpins H1 and H2, and forming along-chain double-chain DNA at a sensing site. Through biotin-streptavidin specific binding reaction, a biotinylated quantum dot CdS is fixed to a sensing site. CdS is initiated by Ag<+> to generate cation exchange reaction, and the fluorescence of Rhod-5N is greatly enhanced by Cd2<+>. Array point fluorescence is quantified by using a gene chip scanner, so that high-sensitivity and high-specificity analysis of target molecules is realized. The method is simple to operate, low in cost, high in sensitivity, good in specificity and high in universality.