A method for reducing quantification errors caused by an optical artefact in digital polymerase chain reaction

[0008] In a first aspect, the present invention relates to a method for reducing quantification errors caused by optical artifacts in a digital polymerase chain reaction (dPCR), wherein the amount or concentration of a nucleic acid of interest is quantified in an array of reaction regions , the method consists of

[0009] a) providing an array of reaction regions used in dPCR;

[0010] b) determining the distribution of the optical signal in each reaction zone;

[0011] c) if the optical signal in the reaction region determined in step b) is not uniformly distributed in the reaction region

[0012] If not, identify the reaction zone as invalid; and

[0013] d) Eliminating reaction regions identified as invalid from the calculation of the amount or concentration of the nucleic acid of interest.

[0014] As detailed above, methods for the reliable determination of the amount or concentration of nucleic acids are of particular importance in several industrial applications, for...