5 results about "Nucleic acid detection" patented technology

The invention discloses a quantitative detection method for FOC (Fusarium oxysporum f. sp. cubense (E.F. Smith) Snyd. & Hans.) race 4 from the soil. The method comprises the following steps: pretreating a sample to be detected and extracting DNA of the sample to be detected; preparing specific primers; conducting fluorescent nucleic acid isothermal amplification detection technique RealAmp reaction; constructing a RealAmp standard curve; and determining the result. The real-time fluorescent nucleic acid isothermal amplification detection technology provided by the invention is a novel nucleic acid detection technology combining a new generation of isothermal nucleic acid amplification technology with the real-time fluorescent detection technology, and has the advantages of high sensitivity, high specificity, low pollution and stable reaction. RealAmp can quantitatively detect pathogens; because of the characteristics of LAMP, Bst DNA polymerase has chain exchange capacity and good tolerance of inhibiting substances contained in the soil DNA extracts; the method carries out detection work on the soil, avoids cover opening and influence by contaminants and conducts on-site soil sample detection; the method for analysis and determination of reaction products is very simple; therefore, the method is suitable for wide application.

The invention belongs to the technical field of molecular detection, discloses a kit for detecting porcine pseudorabies virus (PRV) by combining a centrifugal micro-fluidic chip with a loop-mediated isothermal amplification (LAMP) technology, and establishes a PRV nucleic acid detection method which is rapid, sensitive, high in accuracy and high in repeatability by combining the micro-fluidic chiptechnology. The micro-fluidic chip detection method is high in specificity, and the lowest detection limit can reach 10<2>copies/[mu]L. According to the micro-fluidic chip kit for detecting porcine pseudorabies virus (PRV), a chip analysis device is small and portable, and the defects of time consumption, labor consumption and high cost of PRV detection can be overcome, so that the detection sensitivity and specificity are improved, the labor and equipment cost is reduced, the detection period is shortened, and the requirements of field detection are met. The rapid detection technology can bepopularized and applied to epidemiological investigation and epidemic situation monitoring of the porcine pseudorabies virus (PRV), and has good practical significance and wide market prospects.

The invention belongs to the technical field of nucleic acid detection, and discloses a primer combination and a kit for identifying an African swine fever virus gene deletion strain and an African swine fever epidemic strain by using a centrifugal microfluidic chip. The kit comprises a microfluidic chip, an isothermal amplification premixed solution, an isothermal amplification enzyme solution and a positive standard. The invention further relates to a detection method adopting the kit. The detection method comprises the steps of coating of a primer composition, extraction of a nucleic acid sample to be detected, LAMP reaction and result interpretation. The detection system can rapidly, conveniently, efficiently, specifically and sensitively identify and diagnose African swine fever gene deletion strain and African swine fever wild strain under isothermal conditions, does not need expensive and complex instruments, provides a novel portable detection platform for epidemiological detection and prevention and control of African swine fever, greatly reduces detection cost, is easy to popularize in a large range, and has broad market prospects.

The invention discloses a method for detecting circulating tumor nucleic acid based on a functionalized black phosphorus biosensor, and belongs to a nucleic acid detection method in the field of biomedicine. According to the technical scheme, the method mainly comprises the following steps of preparing p-nitrobenzene functionalized black phosphorus; then, using the p-nitrobenzene functionalized black phosphorus to detect a single-stranded nucleotide sequence. According to the present invention, the p-nitrobenzene functionalized black phosphorus can improve the ability of black phosphorus to distinguish the single-stranded DNA from the double-stranded DNA, and can realize the rapid and sensitive detection of ctDNA in human serum, and the detection limit can reach 0.5 nM or below. Meanwhile,in human serum, the p-nitrobenzene functionalized black phosphorus disclosed by the invention has very strong specificity on ctDNA, and can also be identified even if a single base is mutated or deleted.

The invention belongs to the technical field of medical biotechnology, and particularly relates to a portable nucleic acid detection box. The portable nucleic acid detection box is internally providedwith a foldable workbench, and a lamp post with a sterilization effect is arranged above the workbench, so that the space is saved, and contamination caused by various viruses in the experiment operation process and contamination caused in the sampling process when a virus is detected are avoided. According to the portable nucleic acid detection box, a foldable supporting structure is arranged onthe lower portion of the workbench and used for reinforcing and supporting the workbench, the the portable nucleic acid detection box is convenient to operate, meanwhile, the space is saved through the foldable design, and the portable nucleic acid detection box is easy to operate and convenient to use.