The invention provides a safety test tooling, a safety test device and an application method thereof, wherein the safety test tooling comprises a tooling plate, a first signal collecting module, a second signal collecting module, a first electricity colleting rail, a second electricity collecting rail, a third electricity collecting rail, a blocking air cylinder and a control module, wherein a connector and a socket are arranged on the tooling plate, the connector is electrically connected with the first electricity collecting rail, the socket is electrically connected with the second electricity collecting rail and the third electricity collecting rail, the first, second and third electricity collecting rails are connected with a safety test instrument, the blocking air cylinder is arranged between the first electricity collecting rail and the second collecting rail, the control module is connected with the first signal collecting module, the second signal collecting module, the blocking air cylinder and the safety test instrument, and in addition, the blocking air cylinder and the safety test instrument are controlled according to signals collected by the first signal collecting module and the second signal collecting module. Through the safety test tooling, the safety test device and the application method, the production efficiency can be improved, the production cost can be reduced, the work load of employees can be reduced, and the potential safety hazards are eliminated.