Application of melatonin to alleviation of tomato pollen abortion due to high temperature induction

[0042] (1) Plant material

[0043] Tomato (Solanum lycopersicum L.cv. Micro-Tom) seeds were soaked in warm water at 65°C to accelerate germination, and then sowed in seedling trays with a mixed substrate of vermiculite and perlite (7:1=v:v). Moisture Condition Water to keep it moist. When the fourth true leaf of the tomato seedlings was fully developed, the root washing operation was carried out, and it was transferred to a 1L black opaque plastic pot (40cm×25cm×15cm) filled with Hoagland nutrient solution for hydroponics. Place 4 tomato seedlings in each plastic pot, fix with sponge. In order to ensure the healthy and normal growth of the plants, the nutrient solution was replaced every 3 days, and oxygenation balls were installed in each plastic pot to supplement enough O 2 . The plant growth conditions are as follows: the photoperiod is 14 / 10h (day / night), the temperature is 25 / 23°C (day / night), the average relative humidity is 80%, and the photosynthetic light quan

[0077] The steps are the same as in Example 1, the application method of the melatonin preparation is changed to the in vitro spraying method, sprayed once 3 days before the high temperature treatment, and the pollen viability and germination are measured according to the step (4) of the embodiment, and the results show that the implementation Example 1 processing effect. Spraying time is in the evening, and the amount of spraying per plant varies with the size of the plant. Spray until all leaves are wet, about 15-50mL, and spray 3 times in total.