The invention discloses a
vibrio parahaemolyticus detection method based on nanogold discoloration response isothermal
nucleic acid amplification. According to the invention,
biotin labeled primers of tlh gen are designed for rapid amplification of the tlh gen by loop-mediated isothermal amplification technology, a lot of
biotin labeled repeated sequences are generated in the amplification process and achieve fast aggregation of
streptavidin modified nanogold, and an intense color change effect is produced to reach the purpose of detection of specific genes. The invention establishes an economical, rapid, accurate, specific, sensitive and simple detection method for
vibrio parahaemolyticus in aquatic products, and improves the detection quality and efficiency of
medical health inspection,
disease control and prevention,
aquatic product processing, import and export inspection and other departments.