4 results about "Tryptophan" patented technology

The invention belongs to the technical field of animal infectious disease prevention and curing, and particularly relates to application of mycoplasma bovis MbovP730 protein in natural infection and vaccine immunity identification. According to the codon preference of escherichia coli, the Mbov_730 gene of M.bovis HB0801 is cloned and is modified, and a mycoplasma bovis tryptophan codon UGA is mutated into a tryptophan codon UGG in the escherichia coli so as to express recombinant mycoplasma bovis MbovP730 protein in the escherichia coli. The nucleotide sequence of the MbovP730 protein is disclosed in SEQ ID NO: 13, and the protein can be used as the antigen protein to be applied to the preparation of a kit for identifying mycoplasma bovis wild strain infection and vaccine strain immune identification.

The invention relates to premixed feed capable of increasing egg yield of laying hens in summer as well as a use method of the premixed feed. The composite premixed feed consists of the following components in parts by weight: 8 to 14 parts of composite vitamin, 30 to 40 parts of composite trace element, 90 to 130 parts of monocalcium phosphate and dicalcium phosphate, 250 to 300 parts of stone powder, 40 to 60 parts of table salt, 12 to 20 parts of choline, 50 to 100 parts of anti-stress functional pack, 20 to 25 parts of sodium sulfate, 25 to 35 parts of methionine, 20 to 30 parts of lysineof 70 percent, 3 to 6 parts of threonine, 5 to 8 parts of tryptophan, 10 to 20 parts of intestinal health regulator, 8 to 15 parts of composite enzyme preparation and 250 to 350 parts of kelp powder.A mode of balancing amino acid is adopted, low protein and high amino acid are adopted, plant fat is added to supplement energy, heat increment is reduced, and the anti-stress functional pack and theintestinal health additive are added, so that the heat stress resistance of the organisms can be significantly improved, and the production performance is continuous and stable in high-temperature season.

The invention relates to a high performance liquid chromatography detection method for tryptophan in fodder and belongs to the technical field of tryptophan detection. The detection method comprises the steps of specially pretreating a sample, preparing HPLC analysis and drawing a tryptophan detection standard curve. The detection method has higher flexibility and accuracy, is low in detection limit, better in repeatability, quick, accurate in qualitativeness and quantitation and higher in recovery rate, can accurately analyze tryptophan in the fodder and can be applied to large-scale sample detection and suitable for standardization.