7 results about "Lysine" patented technology

The invention discloses a 1,3-1,4-beta-glucanase mutant, and belongs to the fields of gene engineering and enzyme engineering. Lysine in the 20th position, 117th position and 165th position of 1,3-1,4-beta-glucanase from Bacillus terquilensis mutates through an overlapping extension PCR method to form serine in order to obtain single mutants K20S, K117S and K165S respectively. The above three mutation sites are integrally mutated to obtain a K20S/K117S/K165S triple mutant enzyme. Four mutant enzymes have higher catalysis activity and better thermal stability. Compared with wild enzymes, the above mutant enzymes are in favor of realizing the industrial application.

The invention discloses a non-resistant low-zinc low-copper non-plasma high-grade piglet creep feed and a preparation method thereof. The creep feed comprises the following raw materials: corn, flour,broken rice, puffed soybean powder, milk powder, imported fish meal or fish peptide protein, fermented soybean meal, yeast hydrolysate, soybean protein concentrate, fat powder, acidifying agent, fishsolubles, egg powder, calcium lactate, calcium dihydrogen phosphate, lysine, methionine, threonine, tryptophan, trace element composite package, coated zinc oxide, vitamin composite package, plant extract composite package, plant essential oil, antibacterial peptide and microecological preparation. The product does not use homologous proteins such as plasma protein powder so as to effectively block the occurrence of homologous diseases. The product uses low zinc and low copper so as to reduce environmental pollution and improve the utilization rate of land. The product is free from antibioticso as to reduce drug residue and to ensure human food health. The preparation method provided by the invention is simple, the raw material sources are wide, and the use effect is good.

The present invention relates to granulocyte colony stimulating factor (G-CSF) modified with Y-shaped branched polyethylene glycol (YPEG-G-CSF) at a specific lysine (Lys 17) and the preparation thereof, as well as the pharmaceutical composition comprising YPEG-G-CSF and use thereof.

The invention provides a process for extracting gama-interferon from pichia pastoris fermentation liquor. The process comprises the steps of putting in a tank, centrifuging, membrane separating, shaping, and spray drying. The shaping step is characterized by adding excipient and a protective agent into the fermentation liquor. The excipient is any one of trehalose, starch, mannitol and maltodextrin; the concentration of the excipient is 5 to 20 percent. The protective agent is any one of soya bean lecithin, lysine, hydroxypropyl methyl cellulose and polyethylene glycol 20000; the concentration of the protective agent is 1 to 8 percent. The method provided by the invention is adopted for extracting the gama-interferon from the pichia pastoris fermentation liquor, and the activity of the gama-interferon can reach 3.23 * 106 to 4.29 * 106 IU/ml.

The present invention relates to a preparation method of fragrance-type tobacco spices, which comprises the following preparation steps: taking the following raw materials for use: blank cigarettes (cut tobacco weight 0.6 g): provided by the Technology Center of Anhui China Tobacco Industry Co., Ltd.; fresh and high-quality Hainan pineapple Provided by Wuxi Chaoyang Group; anhydrous glucose, D-fructose, sucrose, malonic acid, anhydrous ethanol, anhydrous methanol, proline, valine, glycine, threonine, lysine, glutamic acid, Partic acid, alanine, leucine, isoleucine, serine, tyrosine: all are analytically pure, provided by Sinopharm Group Chemical Reagent Co., Ltd.; the fragrance type tobacco flavor prepared by the method of the present invention, except In addition to the obvious burnt sweet aroma, it can also produce a light roasted aroma, dried rose aroma and fruity aroma, and the whole method has simple process steps, can be industrialized and has strong adaptability.