Process for extracting gama-interferon from pichia pastoris fermentation liquor

[0034] Example 1 A process for extracting gamma-interferon from Pichia pastoris fermentation broth

[0035] Extracting gamma-interferon from Pichia pastoris fermentation broth comprises the following steps:

[0036] Step 1 put the tank

[0037] The formula for Pichia pastoris fermentation is 1% Pichia pastoris powder, 2% peptone, 2% glucose, and water to make up the balance; the above percentages are mass percentages; the fermentation liquid obtained after Pichia pastoris fermentation is stored in a fermenter;

[0038] Adjust the pressure of the fermentation tank to 0.03MPa, adjust the pressure of the storage tank to zero, and use the pressure difference to hydraulically ferment the fermented Pichia pastoris into the storage tank.

[0039] Step 2 Centrifuge

[0040] Centrifuge the fermented liquid in the storage tank through a centrifuge, the centrifuge speed is 12000rpm, and the centrifuge capacity is 1.2m 3 / h;

[0041] Described centrifuge is two continuous GQ-105 type t

[0055] Example 2 A process for extracting gamma-interferon from Pichia pastoris fermentation broth

[0056] Extracting gamma-interferon from Pichia pastoris fermentation broth comprises the following steps:

[0057] Step 1 put the tank

[0058] The formula for Pichia pastoris fermentation is 1% Pichia pastoris powder, 2% peptone, 2% glucose, and water to make up the balance; the above percentages are mass percentages; the fermentation liquid obtained after Pichia pastoris fermentation is stored in a fermenter;

[0059] Adjust the pressure of the fermentation tank to 0.05MPa, adjust the pressure of the storage tank to zero, and use the pressure difference to hydraulically ferment the fermented Pichia pastoris into the storage tank.

[0060] Step 2 Centrifuge

[0061] The fermentation liquid in the storage tank is centrifuged through a centrifuge, the centrifuge speed is 13000rpm, and the centrifuge capacity is 1.4m 3 / h;

[0062] Described centrifuge is two continuous GQ-105

[0076] Example 3 A process for extracting gamma-interferon from Pichia pastoris fermentation broth

[0077] Extracting gamma-interferon from Pichia pastoris fermentation broth comprises the following steps:

[0078] Step 1 put the tank

[0079] The formula for Pichia pastoris fermentation is 1% Pichia pastoris powder, 2% peptone, 2% glucose, and water to make up the balance; the above percentages are mass percentages; the fermentation liquid obtained after Pichia pastoris fermentation is stored in a fermenter;

[0080] Adjust the pressure of the fermentation tank to 0.07MPa, adjust the pressure of the storage tank to zero, and use the pressure difference to hydraulically ferment the fermented Pichia pastoris into the storage tank.

[0081] Step 2 Centrifuge

[0082] Centrifuge the fermented liquid in the storage tank through a centrifuge, the centrifuge speed is 15000rpm, and the centrifuge capacity is 1.5m 3 / h;

[0083] Described centrifuge is two continuous GQ-105 type t