High performance liquid chromatography detection method for tryptophan in fodder

[0025] A high-performance liquid chromatography detection method for tryptophan in feed, comprising the following steps:

[0026] A. Sample pretreatment

[0027] The feed samples were pulverized and put into microwave-assisted protein hydrolysis equipment for microwave-assisted protein hydrolysis; the microwave power was controlled at 600 W, and the temperature was controlled to rise from room temperature to 150 °C within 10 minutes, and then keep warm for 20 minutes; after the keep warm, The hydrolyzate is processed for detection by high performance liquid chromatography;

[0028] B. Preparation for HPLC analysis

[0029] Column: Syncronis C 18 Chromatographic column, 250×4.6 mm, 5 µm; flow rate: 1 mL / min; column temperature: 30 ℃; detector: DAD detector, detection wavelength: 220 nm; mobile phase: isocratic elution, 0.05 mol / L phosphoric acid Potassium dihydrogen solution + acetonitrile = 90 + 10 (V + V);

[0030] C, drawing of tryptophan detection standard curve

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[0033] A high-performance liquid chromatography detection method for tryptophan in feed, comprising the following steps:

[0034] A. Sample pretreatment

[0035] The feed samples were pulverized and put into microwave-assisted protein hydrolysis equipment for microwave-assisted protein hydrolysis; the microwave power was controlled at 1000 W, the temperature was controlled to rise from room temperature to 170 °C within 30 minutes, and then kept for 40 minutes; after the heat preservation was completed, The hydrolyzate is processed for detection by high performance liquid chromatography;

[0036] B. Preparation for HPLC analysis

[0037] Column: Syncronis C 18 Chromatographic column, 250×4.6 mm, 5 µm; flow rate: 1 mL / min; column temperature: 30 ℃; detector: DAD detector, detection wavelength: 220 nm; mobile phase: isocratic elution, 0.05 mol / L phosphoric acid Potassium dihydrogen solution + acetonitrile = 90 + 10 (V + V);

[0038] C, drawing of tryptophan detection standar

[0041] A high-performance liquid chromatography detection method for tryptophan in feed, comprising the following steps:

[0042] A. Sample pretreatment

[0043]The feed samples were pulverized and put into microwave-assisted protein hydrolysis equipment for microwave-assisted protein hydrolysis; the microwave power was controlled at 800 W, the temperature was controlled to rise from room temperature to 160 °C within 20 minutes, and then kept for 30 minutes; after the heat preservation was completed, The hydrolyzate is processed for detection by high performance liquid chromatography;

[0044] B. Preparation for HPLC analysis

[0045] Column: Syncronis C 18 Chromatographic column, 250×4.6 mm, 5 µm; flow rate: 1 mL / min; column temperature: 30 ℃; detector: DAD detector, detection wavelength: 220 nm; mobile phase: isocratic elution, 0.05 mol / L phosphoric acid Potassium dihydrogen solution + acetonitrile = 90 + 10 (V + V);

[0046] C, drawing of tryptophan detection standard