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5-aminolevulinic acid producing strain and construction method thereof,Method and kit used for determining human TERT gene rs2735940 site polymorphism

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Digestive enzymes are proteins that break down larger molecules like fats, proteins and carbs into smaller molecules that are easier to absorb across the small intestine. Without sufficient digestive enzymes, the body is unable to digest food particles properly, which may lead to food intolerances.

5-aminolevulinic acid producing strain and construction method thereof

PendingCN113930439AIncrease productionReduce manufacturing costBacteriaMicroorganism based processesAmino-Levulinic AcidEnzyme digestion

The invention discloses a 5-aminolevulinic acid producing strain and a construction method thereof. The construction method comprises the following steps of (1) knocking out pxpR, PoxB, ptsG, sucC, sucD and pta genes in bacillus subtilis to obtain a bacillus subtilis engineering strain; (2) carrying out double enzyme digestion treatment on the plasmid pHT43-J by using Aat II and Sma I, integrating the hemO gene onto the plasmid pHT43-J subjected to double enzyme digestion treatment to obtain a recombinant plasmid pHT43-J-hemO, then carrying out enzyme digestion treatment on the recombinant plasmid pHT43-J-hemO by using XbaI and BamHI, and integrating the rhtA gene onto the recombinant plasmid pHT43-J-hemO subjected to enzyme digestion treatment to obtain a first recombinant expression vector; carrying out double enzyme digestion on the plasmid pHT01 by using BamHI and XbaI, and integrating the hemF gene onto the plasmid pHT01 subjected to double enzyme digestion treatment, so as to obtain a second recombinant expression vector; and (3) introducing the obtained first recombinant expression vector and the obtained second recombinant expression vector into the bacillus subtilis engineering bacteria, and constructing to obtain the 5-aminolevulinic acid producing bacteria. The producing bacteria provided by the invention improves the yield of 5-aminolevulinic acid.

Owner:XINTAI JIAHE BIOTECH CO LTD +1

Method and kit used for determining human TERT gene rs2735940 site polymorphism

InactiveCN105256005AReduce yieldControl characteristicsMicrobiological testing/measurementHuman DNA sequencingEnzyme digestion

The invention discloses a method and a kit used for determining human TERT gene rs2735940 site polymorphism. The method comprises following steps: human genome DNA to be determined is provided; upstream primers and downstream primers used for amplification of sequences near human TERT gene rs2735940 site are provided, wherein the downstream primers possess mismatched base C; the human genome DNA to be determined is taken as a template, and the upstream primers and the downstream primers are used for PCR amplification so as to obtain amplification products containing CYGG or CCYGG segments, wherein Y is used for representing base C or T to be determined on human TERT gene rs2735940 site; a restriction enzyme is provided; the restriction enzyme is used for enzyme digestion of the amplification products so as to obtain corresponding enzyme-digested products; and it is determined that Y is used for representing base C or T based on the enzyme-digested products. The restriction enzyme is a restriction enzyme only used for realizing restriction digestion of one segment selected from CCGG and CTGG, or one segment selected from CCCGG and CCTGG. The method is rapid and reliable, and determination cost is reduced greatly.

Method and kit used for determining human TERT gene rs2735940 site polymorphism

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Owner:ZHENGZHOU UNIV

2 results about "Enzyme digestion" patented technology

5-aminolevulinic acid producing strain and construction method thereof

Method and kit used for determining human TERT gene rs2735940 site polymorphism

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