Highland barley biological control disease-resistant and growth promoting method

[0027] 1) High-density culture of Bacillus subtilis (CICC 10339) producing beta glucanase, the starting density of Bacillus subtilis (CICC 10339) is 1.22×10 6 each / mL, inoculated into liquid medium (glucose 14g / L, beef extract 25g / L, potassium dihydrogen phosphate 2.45g / L, peanut cake powder 10g / L, pH=7.0, sterilized at 121°C for 20min), inoculated 2%, shake culture at 37°C for 24 hours, and the density of the bacterial solution is 2.46×10 9 cfu / mL to obtain a high-density bacterial suspension.

[0028] 2) Cut a total of 100kg of healthy highland barley straw into small sections for natural composting, spray 2L of the high-density bacterial suspension prepared in 1) when the internal temperature of the compost reaches 32°C, continue composting, and compost when the internal temperature of the compost exceeds 43°C Finished, stirred and cooled, and post-fermented in a natural state to become a bio-fertilizer rich in beta-glucanase-producing Bacillus subtilis, that is,

[0038] 1) High-density culture of Bacillus subtilis (CICC 10339) producing beta glucanase, the starting density of Bacillus subtilis (CICC 10339) is 1.80×10 6 each / mL, inoculated into liquid medium (glucose 14g / L, beef extract 25g / L, potassium dihydrogen phosphate 2.45g / L, peanut cake powder 10g / L, pH=7.0, sterilized at 121°C for 20min), inoculated Amount of 3%, shaking culture at 37°C for 24 hours, the density of the bacterial solution was 3.34×10 9 cfu / mL to obtain a high-density bacterial suspension.

[0039] 2) Cut a total of 100kg of healthy highland barley straw into small sections for natural composting, spray 3L of the high-density bacterial suspension prepared in 1) when the internal temperature of the compost reaches 32°C, continue composting, and compost when the internal temperature of the compost exceeds 43°C At the end, stir and let it cool, post-ferment in a natural state, and become a bio-fertilizer rich in beta-glucanase-producing Bacillus subtilis.

[0046] 1) High-density culture of Bacillus subtilis (CICC 10339) producing beta glucanase, the starting density of Bacillus subtilis (CICC 10339) is 1.68×10 6 each / mL, inoculated into liquid medium (glucose 14g / L, beef extract 25g / L, potassium dihydrogen phosphate 2.45g / L, peanut cake powder 10g / L, pH=7.0, sterilized at 121°C for 20min), inoculated The amount is 3%, shake culture at 37°C for 24 hours, and the density of the bacterial solution is 2.33×10 9 cfu / mL to obtain a high-density bacterial suspension.

[0047] 2) Cut a total of 100kg of healthy highland barley straw into small sections for natural composting, spray 3L of the high-density bacterial suspension prepared in 1) when the internal temperature of the compost reaches 32°C, continue composting, and compost when the internal temperature of the compost exceeds 43°C At the end, stir and let it cool, post-ferment in a natural state, and become a bio-fertilizer rich in beta-glucanase-producing Bacillus subti