Achromobacter sp. for degrading lignin and application of achromobacter sp. in lignin-containing wastewater treatment

[0021] Embodiment 1: Achromobacter ( Achromobacter sp. ) Isolation and domestication of CCZU-WJ6

[0022] Achromobacter of the present invention ( Achromobacter sp. ) The separation and domestication process of CCZU-WJ6 is as follows:

[0023] 1) Sample, the sample is rotten wood in nature;

[0024] 2) Medium

[0025] Primary screening medium: alkaline lignin medium. Alkaline lignin medium (g / L): Alkaline lignin 2, ammonium sulfate 1.33, magnesium sulfate 0.5, potassium dihydrogen phosphate 1, disodium hydrogen phosphate 0.2, agar 20, pH7.0.

[0026] Re-screening medium: aniline blue solid medium. Aniline blue solid medium (g / L): peptone 10, beef extract 5, sodium chloride 10, aniline blue 0.08, pH 7.0.

[0027] Enrichment medium: LB medium. Enrichment medium (g / L): peptone 10, yeast extract powder 5, sodium chloride 10, pH 7.0.

[0028] 3) Primary screening, secondary screening and fermentation culture operation

[0029] Primary screening: put 5 g of rotten wood i

[0034] Example 2: Preparation of wet cells of Achromobacter sp. CCZU-WJ6

[0035] Prepare the components of the activation medium according to the following concentrations: yeast extract powder 5g / L, peptone 10 g / L, NaCl 10 g / L, pH natural, sterilized at 121°C for 20 minutes, cooled after sterilization, and inserted into the slope Seed (the achromobacter that prepares in embodiment 1 ( Achromobacter sp. ) CCZU-WJ6), cultivated for 1 day at 30°C with a shaker speed of 250r / min, and used it as an activation culture seed solution.

[0036] Prepare the components of the fermentation medium according to the following concentrations: sucrose 20 g / L, peptone 10 g / L, NaCl 10 g / L, pH 7.0, sterilized at 121°C for 20 minutes, cooled after sterilization, according to the volume ratio of inoculum 10% was inoculated with the activated culture seed solution, and cultured for 1 day at a temperature of 30°C and a shaker speed of 180 r / min to obtain a fermentation culture solution

[0037] Example 3: Preparation of wet cells of Achromobacter sp. CCZU-WJ6

[0038] Same as Example 2, the difference is that the components of the fermentation medium are prepared according to the following concentrations: yeast extract powder 5 g / L, peptone 10 g / L, NaCl 10 g / L, pH natural.