Nitrite-degrading electron donor protein obtained from lactobacillus casei and preparation method thereof and application

[0035] Example 1

[0036] 1. A preparation method of Lactobacillus casei degrading electron donor protein for nitrite, including the following steps and process conditions:

[0037] (1) Place the freeze-dried powder of Lactobacillus casei subsp. rhamnosus in MRS medium and culture it at 37°C for 24 hours to prepare seed liquid; the described Lactobacillus casei subsp. rhamnosus is purchased from China Industrial Microbial Culture Collection Management Center, numbered CICC6013, named Lactobacilluscaseisubsp.rhamnosus6013 (abbreviated as LCR6013).

[0038] (2) Inoculate LCR6013 seed liquid into induction medium A at 5% by volume, and culture it at 37°C for 24 hours to obtain induction working starter. The formulation of the induction medium A is: containing the inducer NaNO 2 MRS medium with a concentration of 10μg / mL.

[0039] (3) Inoculate the induction working starter into induction medium B at a volume percentage of 5%, statically induce culture at 37°C for 24 hours, and contain a

[0050] Example 2

[0051] 1. A preparation method of Lactobacillus casei degrading electron donor protein for nitrite, including the following steps and process conditions:

[0052] (1) Put the freeze-dried powder of Lactobacillus casei subsp. rhamnosus into MRS medium, and then culture it at 30°C for 36 hours to prepare seed liquid.

[0053] (2) Inoculate LCR6013 seed solution into induction medium A at a rate of 10% by volume, and culture it for 18 hours at 40° C. to obtain induction working starter. The formulation of the induction medium A is: containing the inducer NaNO 2 MRS medium with a concentration of 5μg / mL.

[0054] (3) Inoculate the induction working starter into induction medium B at a volume percentage of 15%, statically induce culture at 30°C for 24 hours, and contain a culture medium containing electron donor protein that degrades nitrite. The formulation of the induction medium B is: containing the inducer NaNO 2 MRS medium with a concentration of 20μg / mL.

[0055]

[0065] Example 3

[0066] 1. A preparation method of Lactobacillus casei degrading electron donor protein for nitrite, including the following steps and process conditions:

[0067] (1) Place the lyophilized powder of Lactobacillus casei subsp. rhamnosus in MRS medium and culture it at 37°C for 24 hours to prepare seed liquid;

[0068] (2) Inoculate LCR6013 seed liquid into induction medium A at a rate of 1% by volume, and culture it for 24 hours at 40°C to obtain induction working starter. The formulation of the induction medium A is: containing the inducer NaNO 2 MRS medium with a concentration of 5μg / mL.

[0069] (3) Inoculate the induction working starter into induction medium B at a volume percentage of 10%, statically induce culture at 30°C for 24 hours, and contain a culture solution containing electron donor protein that degrades nitrite. The formulation of the induction medium B is: containing the inducer NaNO 2 MRS medium with a concentration of 5μg / mL.

[0070] (4) Centrif