Chicken Flavivirus and inactivated vaccine thereof

[0012] Chicken yellow virus FQ-C1 strain of the present invention is obtained in the following manner:

[0013] In December 2010, a 210-day-old laying hen flock in a laying hen farm in Fuqing City experienced an acute and severe drop in egg production, but no chickens died, and the use of antibacterial and antiviral drugs had no obvious effect. We isolated a strain of virus from the tissues of dead diseased laying hens, and ruled out avian influenza virus, chicken egg drop syndrome virus, chicken Newcastle disease virus, chicken infectious bronchitis virus, chicken infectious laryngotracheitis through laboratory testing Viruses, chicken arthritis virus, chicken infectious bursal virus, through flavivirus-specific RT-PCR identification and sequencing analysis, it was shown that the FQ-C1 strain virus belongs to the Flavivirus genus of the family Flaviviridae.

[0014] (1) Treatment of diseased materials: Aseptically collect hemorrhage follicles from laying hens showing abnormal eg

[0021] Chicken yellow virus inactivated vaccine of the present invention is obtained in the following manner:

[0022] Chicken yellow virus FQ-C1 strain is inoculated into SPF chicken embryo, collects dead SPF chicken embryo allantoic fluid after inoculation, wherein the content of chicken yellow virus FQ-C1 strain is 10 3.33 ELD 50 / 0.2ml, SPF chicken embryo allantoic fluid is inactivated by formaldehyde and added white oil adjuvant to emulsify, and is prepared into a conventional oil emulsion inactivated vaccine.

[0023] The immune result of the prepared inactivated vaccine is as follows:

[0024] 1. Vaccine safety test

[0025] 1.1 Safety test of SPF chicks

[0026] 60 1-day-old SPF chicks were purchased, and they were randomly divided into 4 groups when they were 7 days old, with 15 chickens in each group. The SPF chickens in the 3 experimental groups were injected intramuscularly with different batches (3 batches) of chicken yellow virus through the legs. The inactiv