Streptomyces bacterial strain, and culture method and applications thereof

[0020] The isolation and identification of embodiment 1 Streptomyces bacterial strain (Streptomyces)

[0021] 1. Experimental materials and conditions

[0022] (1) Soil samples collected in a certain place in Chenzhou City, Hunan Province.

[0023] (2) The components per liter of the liquid medium are as follows: 3.0 g of beef extract, 10.0 g of peptone, 5.0 g of sodium chloride, dilute to 1 liter of water, and the pH is 7.4-7.6.

[0024] The components per liter of solid medium are as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, agar 15-25g, water to 1 liter, pH 7.4-7.6.

[0025] The culture medium was divided into aliquots and sterilized in an autoclave (121°C, 30min).

[0026] (3) Sterile operating conditions: All utensils and utensils must be sterilized in an autoclave (121°C, 30min), and operations such as inoculation are carried out in ultra-clean workbenches.

[0027] (4) Strain culture conditions: culture in a light incubator (14L: 10D) at 25±1°C, t

[0042] Example 2 Application of Streptomyces strain "TY-001#" in the preparation of microbial organic fertilizers with high osmotic pressure resistance and aerated fermentation

[0043] (1) a preparation method of microbial organic fertilizer, comprising the following steps:

[0044] Weigh 100g of soybeans, soak them in water for 10 hours, put them into a refiner and grind them into soybean milk, filter out the bean dregs, and place the soybean milk in a clean fermenter. Soak the bean dregs in water, filter again, and mix the filtrate with soybean milk evenly.

[0045] Add 100g of white granulated sugar to the fermenter, stir to dissolve the white granulated sugar completely, and then set the volume to 1L. The white granulated sugar concentration in the medium is as high as 100g / L, which belongs to the high osmotic pressure medium.

[0046] Inoculation. Take 60mL of "TY-001#" liquid spawn (200 million live bacteria / mL), add it to the fermenter, and stir evenly.

[0047] f