Preparation method of buckwheat protein biological activity peptides

[0018] 1) Dissolve buckwheat protein in NaOH solution of pH 6.0 to make the protein concentration reach 15g / L;

[0019] 2) At 40°C, add 4000U / g alkaline protease, hydrolyze for 2h, and the degree of hydrolysis is 40%;

[0020] 3) After the hydrolyzed protein peptide solution was incubated in a 95° C. water bath for 10 min to inactivate the enzyme, centrifuged at 10,000 rpm for 40 min, and the clear liquid was vacuum freeze-dried to obtain dry powder of buckwheat protein bioactive peptide.

[0022] 1) Dissolve the buckwheat protein in the NaOH solution of pH 8.0 to make the protein concentration reach 18g / L;

[0023] 2) At 30°C, add 5000U / g alkaline protease, hydrolyze for 1.5h, and the degree of hydrolysis is 30%;

[0024] 3) After the hydrolyzed protein peptide solution was incubated in a 97° C. water bath for 10 min to inactivate the enzyme, centrifuged at 10,000 rpm for 40 min, and the clear liquid was vacuum freeze-dried to obtain dry powder of buckwheat protein bioactive peptide.

[0026] 1) Dissolve buckwheat protein in NaOH solution of pH 12.0 to make the protein concentration reach 21g / L;

[0027] 2) At 50°C, add 6500U / g alkaline protease, hydrolyze for 3h, and the degree of hydrolysis is 55%;

[0028] 3) After the hydrolyzed protein peptide solution was incubated in a 96° C. water bath for 10 min to inactivate the enzyme, centrifuged at 10 000 rpm for 40 min, and the clear liquid was vacuum freeze-dried to obtain dry powder of buckwheat protein bioactive peptide.