3 results about "Elisa method" patented technology

The invention provides a preparation method of monoclonal antibodies for IC (nfectious coryza) of chickens. According to the technical scheme, hybridoma cell strains secreting the monoclonal antibodies against avibacterium paragallinarum are prepared from B type strains, the secreted antibodies are only targeted at the B type strains and do not have reactions with A type and C type avibacterium paragallinarum, and accordingly, the antibodies are only targeted at B type serotype diseases. Besides, an ELISA (enzyme-linked immunosorbent assay) method and an immune colloidal gold method established according to the characteristics of the monoclonal antibodies have good specificity and sensitivity and can be used for clinical detection. Besides, the monoclonal antibodies prepared with the method are high in titer, better in specificity and especially applicable to further preparation of B type avibacterium paragallinarum antigen diagnostic kits or B type avibacterium paragallinarum antigen diagnostic colloidal gold test paper. Prominent technical effects are realized through innovative technical improvements, and the preparation method is lower in cost and easy to implement and has prominent popularization prospect.

The invention belongs to the field of antibody engineering, and particularly relates to a stably expressed anti-interferon gamma (IFN-gamma) genetically engineered single-chain antibody strain and application. A gene of an interferon gamma is cloned in vitro through a genetic engineering measure to build a prokaryotic expression vector; isopropyl thiogalactoside (IPTG) induction, expression and purification are performed to obtain soluble interferon antigen protein. The anti-(IFN-gamma) genetically engineered single-chain antibody strain capable of realizing stable secretory expression is selected by using a phage display technology, and a functional single-chain antibody is expressed and purified by building the prokaryotic expression vector; by use of a competitive enzyme-linked immunosorbent assay (ELISA) method, qualitative and quantitative detection for IFN-gamma in human body is realized; a foundation is laid for commercial development and production of a high-quality enzyme-linked immunosorbent assay kit and a gold-labelled antibody detection card.