Dry chemical reagent tablet for quantitatively measuring concentration of total cholesterol and preparation method of dry chemical reagent tablet

[0069] Embodiment 1: the preparation method of total cholesterol dry chemical reagent sheet of the present invention

[0070] The transparent support layer is made of polyethylene terephthalate, with a thickness of 0.178 mm, a length of 30 cm, and a width of 20 cm. The surface is treated with ultraviolet irradiation, the irradiation distance is 10cm, and the irradiation time is 60 minutes.

[0071] The formula of chromogenic solution reagent is as follows:

[0072]

[0073] The formula of reaction liquid reagent is as follows:

[0074]

[0075] The color-developing layer is coated on the ultraviolet-treated polyethylene terephthalate support layer, and the reagent layer is continuously coated on the color-developing layer, and put into a drying box for drying at 30°C for 20 minutes.

[0076] The formula of the diffusion liquid reagent is as follows:

[0077]

[0078]

[0079] Spray the diffusion liquid evenly on the reagent layer, and put it in a drying oven to dry

[0081] Embodiment 2: the accuracy analysis of the method of the present invention

[0082] Test samples: blood samples from 20 medical examiners;

[0083] Comparative detection method: use the commercially available total cholesterol detection kit (GOD-PAP method) 4:1 liquid reagent to detect on the Hitachi 7080 automatic biochemical analyzer, add 2.0 μL sample to the automatic biochemical analyzer, reagent one 160 μL, reagent 40 μL, react at 37° C. for 10 minutes, measure the absorbance value at 590 nm, and calculate the concentration of total cholesterol in the sample according to the standard curve.

[0084] 20 samples were measured respectively with the detection method of Example 1 and the comparative detection method, and the detection results are shown in Table 1.

[0085] Table 1 analysis results

[0086]

[0087] The results show that the R calculated according to the test results 2 The value is 0.9933, which is greater than 0.95, showing that the detection result

[0088] Embodiment 3: the precision analysis of the method of the present invention

[0089] Test sample: any serum sample;

[0090] Using the detection method of Example 1, the same sample to be tested was repeatedly detected 10 times, and the detection results are shown in Table 2.

[0091] Table 2 detects sample total cholesterol concentration (10 times) and standard deviation rate (variation coefficient)

[0092]

[0093] The results show that the standard deviation rate is 1.88%, which is less than 10% of the standard requirement, indicating that the method of the present invention has high precision.