Test strip for detecting organophosphorus pesticide and application thereof

[0035] The preparation of embodiment 1 organophosphorus pesticide detection test strip

[0036] The preparation method of this test strip mainly comprises the following steps:

[0037] 1) Prepare a conjugate release pad sprayed with an organophosphorus pesticide monoclonal antibody-colloidal gold marker;

[0038] 2) preparing a reaction membrane with a detection line coated with an organophosphorus pesticide hapten-carrier protein conjugate and a quality control line coated with a goat anti-mouse anti-antibody;

[0039] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the PVC bottom plate into a test strip.

[0040] The following step-by-step detailed description:

[0041] 1. Preparation of organophosphorus pesticide hapten

[0042] (1) Take 1.0g of compound a, add 30ml of methanol to dissolve, take 0.27g of hydroxylamine hydrochloride, add 1ml of water to dissolve, add to the methanol s

[0077] The detection of organophosphorus pesticide residues in the sample of embodiment 2

[0078] 1. Test with test strips

[0079] Use a straw to absorb the sample solution to be tested and add 3 drops vertically to the sample hole, start timing when the liquid flows, react for 5-10 minutes, and judge the result.

[0080] 2. Analyze test results

[0081] Read the result by colloidal gold analyzer (referred to as "analyzer"):

[0082] Negative (-): Indicates that the concentration of the substance to be tested in the sample is lower than the detection limit;

[0083] Positive (+): Indicates that the concentration of the substance to be tested in the sample is equal to or higher than the detection limit;

[0084] Invalid: Indicates that retesting is required.

[0085] Example 3 sample detection example

[0086] 1. Detection limit test

[0087] Take blank cabbage and apple samples, add organophosphorus pesticides to the final concentrations of 0.1, 0.2, and 0.4 mg / kg, respectively, take test strips for detection, and repeat the measurement three times for each sample.

[0088] When testing cabbage and apple samples with test strips, when the added concentration of organophosphorus pesticide is 0.1mg / kg, the analyzer shows negative; when the added concentration of organophosphorus pesticide is 0.2, 0.4mg / kg, the analyzer shows is positive.

[0089]

[0090]

[0091] 2. False positive rate and false negative rate test

[0092] Take 20 positive samples of cabbage and apples with a known organophosphorus pesticide content of 0.2 mg / kg and 20 negative samples of cabbage and apples with a content of less than 0.1 mg / kg, test with three batches of test strips, and calculate the Yin and Yang sex rate. The results are shown in