Preparation and application of placenta acellular matrix gel loaded with living cells

A technology of acellular matrix and living cells, which is applied in the preparation and application of placental acellular matrix gel, which can solve the problems of inability to directly serve as a carrier to load cells, difficulty in removing glutaraldehyde, and product denaturation, so as to improve vitality and death The efficiency, reagents and methods are simple, and the effect of gelation time is short

Inactive Publication Date: 2022-04-12
杭州倍荣生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology describes how we use special chemicals called placentae extract (PSE), an ingredient found naturally within certain types of animal tissues like cow or sheep' s milk, to create matrices made from these sources with unique properties. These compositions have been shown safe even without any harmful compounds added during processing. They also provide various benefits including enhanced regulation over gene expression levels and reduced costs compared to traditional methods involving expensive agents.

Problems solved by technology

This patents discusses how porous cell matrices made up of different types of fibers have potential applications in various medical fields such as wound healing or dentistry. However current techniques involve physically removing unwanted components like viruses during manufacturing processes, making these structures less effective than desired. Therefore, this technical problem addressed in this patented text relates to developing new ways to make them stronger without relying solely upon chemotherapy treatments.

Method used

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  • Preparation and application of placenta acellular matrix gel loaded with living cells
  • Preparation and application of placenta acellular matrix gel loaded with living cells
  • Preparation and application of placenta acellular matrix gel loaded with living cells

Examples

Experimental program
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Embodiment 1

[0041] A kind of preparation of placenta decellularized matrix gel loaded with living cells, the steps are as follows:

[0042] (1) Material selection of placenta tissue: fresh placenta delivered within 6 hours.

[0043] (2) Placental tissue collection and transportation: collect fresh placenta, wash it with water to remove blood stains, and then immerse it in a sterile preservation solution containing peracetic acid and ethanol. After soaking in this solution for 1 hour, place the placenta tissue in -8 Store below ℃ for a long time. The placenta is transported in cold chain at low temperature (<0°C).

[0044] (3) Placental tissue virus inactivation treatment: the virus inactivation solution is mixed with peracetic acid, absolute ethanol and purified water in equal volume ratios. Add 20L of virus inactivation solution to 1kg of placenta tissue and treat it for 6 hours.

[0045] (4) Removal of virus inactivation solution residues: centrifuge the inactivated placenta supernatant

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PUM

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Abstract

The invention discloses a preparation method of placenta acellular matrix gel loaded with living cells, which comprises the following steps: pretreating placenta tissues, carrying out virus inactivation treatment on the placenta tissues, removing residues of a virus inactivation solution, carrying out three-time circulating freeze thawing treatment on the tissues, carrying out acellular treatment, removing residues of an acellular solution, drying and ball-milling the tissues, and carrying out irradiation sterilization. Adding normal saline into the sterilized powder, dissolving, adding extracted living cells, uniformly mixing, placing in different molds, and placing in an incubator at 37 DEG C for 10-20 minutes to form gel. According to the preparation and application of the placenta acellular matrix gel loaded with the living cells, the prepared gel can load various cells and support the growth of the cells, the gel forming time is short, and the activity and death rate of the cells can be improved.

Description

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Claims

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Application Information

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Owner 杭州倍荣生物科技有限公司
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