Pleurotus nebrodensis liquid strain culture medium and propagation method of strain

[0022] Example 1

[0023] An edible fungus production cooperative has 500L liquid strain fermentation equipment. The method for producing white spirit mushroom liquid strain culture medium and strain propagation is as follows;

[0024] (1) First weigh 16 kg of bran, 10 kg of corn flour, 4.8 kg of soybean meal powder, 8 kg of glucose, 0.4 kg of peptone, and 0.48 kg of KH. 2 PO 4 , 0.2 kg of MgSO 4 , 0.04 kg of VB 1 , According to the weight, add 400L of water in order from less to more during stirring, adjust the pH of the mixed solution to 6, then transfer it to the liquid strain fermentation tank, sterilize, when the sterilized medium liquid is cooled to After the room temperature, the purchased solid Pleurotus ostreatus seeds are connected, and then the culture liquid is adjusted to 24-25°C, the aeration pressure is ≥0.3Kg / cm, and the fermentation culture is 7 days to obtain the Pleurotus ostreatus liquid culture species.

[0025] (2) While preparing the liquid culture strains, prep

[0026] Example 2

[0027] A farmer has a 100L liquid strain fermentation equipment, and the method for making liquid culture medium for Pleurotus ostreatus and strain propagation is as follows;

[0028] (1) First weigh 4.5 kg of bran, 2 kg of corn flour, 1.6 kg of soybean meal powder, 1.5 kg of glucose, 0.12 kg of peptone, and 0.1 kg of KH. 2 PO 4 , 0.06 kg of MgSO 4 , 0.01 kg of VB 1 , According to the weight, add 100L of water while stirring from as little as as possible, adjust the pH of the mixed solution to 6.5, then transfer it to the liquid fermentation tank for sterilization, when the sterilized medium liquid is cooled to After room temperature, insert the purchased solid seeds of Pleurotus ostreatus, and then adjust the culture liquid to 24-25°C, with aeration pressure ≥ 0.3Kg / cm, and fermentation and culture for 8 days to prepare Pleurotus ostreatus in liquid culture.

[0029] (2) While preparing liquid culture strains, prepare the solid bacterial bag medium ingredients, a