Methods of using sulfur nucleophiles as improved alternatives to sodium bisulfite for methylated DNA analysis
a technology of methylated dna and sulfur nucleophiles, which is applied in the field of sulfur nucleophiles, can solve the problems of inability to detect methylated dna, long reaction time and tedious clean-up procedures, and the current use of sodium bisulfite protocols is plagued by incomplete conversion, irreproducible results,
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[0035] It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention. In this application, the use of the singular includes the plural unless specifically stated otherwise. In this application, the use of “or” means “and / or” unless stated otherwise. The use of the term “comprising,” as well as other forms, such as “comprises” and “comprise,” will be considered inclusive, in that the term “comprising” leaves open the possibility of including additional elements. Furthermore, the use of the terms “including” or “having”, as well as other forms, such as “includes”, “has”, “included”, and “have” is not intended to be limiting. Also, terms such as “element” or “component” encompass both elements and components comprising one unit and elements and components that comprise more than one subunit unless specifically stated otherwise.
[0036] The section headings used herein a
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