Methods of using sulfur nucleophiles as improved alternatives to sodium bisulfite for methylated DNA analysis

a technology of methylated dna and sulfur nucleophiles, which is applied in the field of sulfur nucleophiles, can solve the problems of inability to detect methylated dna, long reaction time and tedious clean-up procedures, and the current use of sodium bisulfite protocols is plagued by incomplete conversion, irreproducible results,

Inactive Publication Date: 2006-03-23
APPL BIOSYSTEMS INC
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AI Technical Summary

Benefits of technology

The technical effect of this invention relates to improving methods for detecting changes caused by genetic material on DNA (mDNA). This would allow researchers more accurate understanding about how certain genes are being affected or changed during various stages of development such as cell division.

Problems solved by technology

There have been various technical problem addressed during studying how accurately detect changes made from cell cycle regulation caused by modifications like rearrangements within specific regions called coding region(CN)-associating sequences. Current assay techniques involve complicated steps involving multiple chemical processes followed by washing operations before measuring the modified nucleus' content. Additionally, existing approaches suffer from poor reproducibility due to variations between different laborator workers who perform them manually. Therefore, there needs better ways to convert cytoferon into urancytide without compromising accuracy.

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  • Methods of using sulfur nucleophiles as improved alternatives to sodium bisulfite for methylated DNA analysis
  • Methods of using sulfur nucleophiles as improved alternatives to sodium bisulfite for methylated DNA analysis
  • Methods of using sulfur nucleophiles as improved alternatives to sodium bisulfite for methylated DNA analysis

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[0035] It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention. In this application, the use of the singular includes the plural unless specifically stated otherwise. In this application, the use of “or” means “and / or” unless stated otherwise. The use of the term “comprising,” as well as other forms, such as “comprises” and “comprise,” will be considered inclusive, in that the term “comprising” leaves open the possibility of including additional elements. Furthermore, the use of the terms “including” or “having”, as well as other forms, such as “includes”, “has”, “included”, and “have” is not intended to be limiting. Also, terms such as “element” or “component” encompass both elements and components comprising one unit and elements and components that comprise more than one subunit unless specifically stated otherwise.

[0036] The section headings used herein a

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Abstract

The invention provides for the use of sulfur nucleophiles in analyzing methylated DNA and novel sulfur nucleophiles suitable for such us.

Description

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Claims

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Application Information

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Owner APPL BIOSYSTEMS INC
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