2 results about "Precursor cell" patented technology

The invention discloses an in-vitro cochlea micro-organ functional unit and a three-dimensional construction method and application thereof. Separated single mouse cochlear precursor cells are subjected to three-dimensional induction culture to form cochlear organs, the cochlear organs and spiral neuron tissues are subjected to three-dimensional co-culture, and meanwhile, the cochlear organs are induced to be differentiated and mature into hair cells and promote growth of spiral neurons, so that a cochlear micro-organ functional unit with synaptic connection between hair cells and spiral neurons is constructed. The cochlear micro-organ functional unit provides a new thought and a good application model for auditory function reconstruction, and is used for researching a regulation and control mechanism of functional synapses formed by regenerated hair cells and spiral neurons; and a series of problems such as an inner ear development mechanism, a hair cell and spiral ganglion neuron damage mechanism and intervention measures, hair cell regeneration regulation and control and auditory function reconstruction are better researched.

The invention relates to a method for purifying fat cells and precursor cells thereof. From fat tissue blocks of a human body or a mammal, after cleaning is carried out by mixed solution of a red blood cell lysis buffer and DMEM F / 12, digestion is carried out by adopting tissue digestive juice, and after the steps of centrifugation, filtering processing and the like are carried out, the high-purity and high-activity fat-derived precursor cells can be efficiently separated and obtained from trace of fat tissues; separated fat-derived precursor cell culture solution has different types of cell nutritional factors with high concentration; and the obtained fat-derived precursor cells and a product thereof can be used for tissue repair.