5 results about "Red blood cell" patented technology

Disclosed are compounds having the structure:

wherein Z is —OH or —O•; and

A is:

wherein

• • • X and Y are independently NR₁ or O, where
    • R₁ is H or C₁-C₄ alkyl; and
    • R₂ is H, C₁-C₄ alkyl or t-butoxycarbonyl,
    • wherein W is C₃-C₄ alkynyl; and
    • R₁ is H or C₁-C₄ alkyl, or
    • wherein R₁ is H, C₁-C₄ alkyl, or C₃-C₄ alkynyl; and
      • R₃ is H, OH, O(C₁-C₄ alkyl), or a halogen,

optically active enantiomers, pharmaceutically acceptable salts of the compounds, pharmaceutical compositions containing such compounds or salts, and processes for their preparation. The subject invention also provides methods of alleviating symptoms of neurologic, autoimmune, and inflammatory disorders caused by the presence of reactive oxygen species, methods of preventing oxidation of lipids, proteins, or deoxyribonucleic acids on a cellular level, and methods of protecting human red blood cells from lysis by O₂ radicals.

The invention discloses an application of porphyrin in tin in preparation of medicines for treating thalassemia, which uses porphyrin in tin for intraperitoneal injection of beta thalassemia mice, and finds that porphyrin in tin can enable invalid red systems of thalassemia mice to return to normal, so that alpha/beta globin chain imbalance of red blood cells is effectively relieved, and the curative effect of thalassemia is improved. The red blood cell life, the total red blood cell number and the hemoglobin level are improved; meanwhile, the iron content and splenomegaly of serum and liver are reduced. After porphyrin in tin is used for treatment, invalid hematopoiesis, anemia and iron overload of thalassemia mice are effectively relieved, no obvious toxic or side effect exists, treatment is convenient to implement, and the curative effect can be kept only by maintaining low dosage after high dosage.

The invention provides a preparation method of mouse bone marrow macrophages. The preparation method comprises the steps as follows: (1) preparing a complete medium; (2) dislocating cervical vertebraof mice of 6-9 weeks old; (3) taking femur and tibia of the mice, blowing out mouse bone marrow cells, repeating the operation until the bone is colorless, collecting a single cell suspension after blowing, performing centrifuging, and discarding a supernatant; (4) adding a red blood cell lysis buffer to cell precipitate for treatment; (5) adding the mixed solution to a complete medium rapidly forneutralization, performing centrifuging, and discarding a supernatant; (6) adding the complete medium to centrifugal cell precipitate to resuspend cells; (7) inoculating a petri dish with the resuspended cells for culture; (8) after 4 hours, sucking out the suspension cells, inoculating a petri dish of the complete medium containing an inducer with the cells, and recording the day as the first day; (9) changing half of the medium of the cells on the third day, changing the medium every other day until the seventh day, and performing testing. A simple, economical and rapid method for separating the mouse bone marrow macrophages is provided.