Method for purifying fat cells and precursor cells thereof and application thereof

[0029] 1) Obtain 0.3cm from the human skin 3 The adipose tissue block was labeled as human adipose tissue 001, resuspended in DMEM F / 12, and placed in a 4°C refrigerator for processing.

[0030] 2) Before the separation of adipose-derived precursor cells, the adipose tissue was rinsed with 75% alcohol for 1 second, and the outer packaging was wiped clean.

[0031] 3) The operation was performed in a biological safety cabinet, and the adipose tissue was washed with normal saline for at least 3 times in sequence.

[0032] 4) Discard the normal saline, move the adipose tissue to a clean culture dish, add 1mL of red blood cell lysate and DMEM F / 12 mixed solution, wash the adipose tissue 3 times, 5 minutes each time, and cut up the adipose tissue; The mixing ratio of lysate and DMEM F / 12 is 1:1 by volume.

[0033] 5) According to 10 times the volume, use the mixture of red blood cell lysate and DMEM F / 12 to resuspend the chopped adipose tissue, transfer it to a clean centrifuge t...

[0043] 1) Obtain 1 cm from human subcutaneous 3 The adipose tissue block was labeled as human adipose tissue 002, resuspended in DMEM F / 12, and placed in a 4°C ice box for processing.

[0044] 2) Before the separation of adipose-derived precursor cells, the adipose tissue was rinsed with 75% alcohol for 30 seconds, and the outer packaging was wiped clean.

[0045] 3) The operation was performed in a biological safety cabinet, and the adipose tissue was washed with normal saline for at least 3 times in sequence.

[0046] 4) Discard the clean normal saline, move the adipose tissue to a clean culture dish, add 5mL of red blood cell lysate and DMEM F / 12 mixed solution, wash the adipose tissue 3 times, 5 minutes each time, and cut up the adipose tissue; red blood cells The mixing ratio of lysate and DMEM F / 12 is 1:1 by volume.

[0047]5) According to 10 times the volume, use the mixture of red blood cell lysate and DMEM F / 12 to resuspend the chopped adipose tissue, transfer it to...

[0057] 1) Obtain 3 cm from monkey skin 3 The adipose tissue block was labeled as monkey-derived adipose tissue 001, resuspended in DMEM F / 12, and placed in a 4°C refrigerator for processing.

[0058] 2) Before the separation of adipose-derived precursor cells, the adipose tissue was rinsed with 75% alcohol for 15 seconds, and the outer packaging was wiped clean.

[0059] 3) The operation was performed in a biological safety cabinet, and the adipose tissue was washed with normal saline for at least 3 times in sequence.

[0060] 4) Discard the clean normal saline, move the adipose tissue to a clean culture dish, add 3 mL of red blood cell lysate and DMEM F / 12 mixed solution, wash the adipose tissue 3 times, 5 minutes each time, and cut up the adipose tissue; The mixing ratio of lysate and DMEM F / 12 is 1:1 by volume.

[0061] 5) According to 10 times the volume, use the mixture of red blood cell lysate and DMEM F / 12 to resuspend the chopped adipose tissue, transfer it to a clea...