2 results about "Adherent Culture" patented technology

The invention discloses a bovine kidney cell capable of being subjected to suspension culture, a suspension culture method for the bovine kidney cell and application thereof. The bovine kidney cell, the suspension culture method for the bovine kidney cell (MDBK) and the application have the advantages that a large quantity of manual operation for cell digestion, liquid change, frequent seed separation and the like can be omitted by the suspension culture method, the suspension culture method is simple, convenient and speedy, processes can be automated by the aid of reactor culture, the cell yield of the unit volume can be increased, and the technical difficult problem of difference between the traditional adherent culture cell batches can be solved; the bovine kidney cell (MDBK) and suspension culture cells of the bovine kidney cell can be used in the fields of culture of diversified sensitive viruses, production of antibody proteins, production of vaccine, examination of exogenous viruses of products and the like.

The invention provides an integrated bioreactor for adherent cell culture. The bioreactor comprises a regulation and control system, a reaction vessel body and a cell culture unit which are sequentially connected and internally communicated with one another; the regulation and control system is provided with a gas pressure balance hole and a liquid dropping hole, the cell culture unit is providedwith a central flow guiding hole and a liquid level monitoring hole, and the gas pressure balance hole and the liquid level monitoring hole are communicated with each other through a pipeline to forma liquid level sensor; the bioreactor further comprises a cell suspension bottle, one end of the central flow guiding hole is communicated with the liquid dropping hole through a pipeline, and the other end of the central flow guiding hole is communicated with the cell suspension bottle through a pipeline. The integrated bioreactor successfully exchanges and circulates culture mediums of the cellculture unit and the regulation and control system, and the unified regulation and control over the pH value and the dissolved oxygen value of each unit culture medium are successfully achieved; the laminar flow shear force and liquid level of the cell culture unit are also regulated and controlled.