Preparation method of mouse bone marrow macrophages

[0023] The invention provides a method for preparing mouse bone marrow macrophages, comprising the following steps:

[0024] (1) preparing a DMEM medium containing 10% fetal bovine serum to obtain a complete medium, and putting the mouse fibroblast L929 cell culture supernatant and the complete medium into a water bath for incubation;

[0025] (2) Mice 6-9 weeks old were killed by cervical dislocation; soaked in 75% ethanol for 2-3 minutes;

[0026] (3) Sterilized surgical scissors to peel off the skin and muscles of the mouse legs, cut the femur and tibia of the mouse, scrape off the muscle and fascia on the bone as much as possible, and use phosphate buffer containing 2% penicillin and streptomycin Fluid rinses the bone surface;

[0027] (4) Cut off both ends of the femur and tibia with surgical scissors; absorb DMEM medium with a 5mL disposable syringe, insert the needle of the syringe into the medullary cavity of the femur and tibia accurately, blow out the bone marrow, repe

[0035] (1) preparing a DMEM medium containing 10% fetal bovine serum to obtain a complete medium, and putting the mouse fibroblast L929 cell culture supernatant and the complete medium into a water bath for incubation;

[0036] (2) Mice 6-9 weeks old were killed by cervical dislocation; soaked in 75% ethanol for 2-3 minutes;

[0037] (3) Sterilized surgical scissors to peel off the skin and muscles of the mouse legs, cut the femur and tibia of the mouse, scrape off the muscle and fascia on the bone as much as possible, and use phosphate buffer containing 2% penicillin and streptomycin Fluid rinses the bone surface;

[0038] (4) Cut off both ends of the femur and tibia with surgical scissors; absorb DMEM medium with a 5mL disposable syringe, insert the needle of the syringe into the medullary cavity of the femur and tibia accurately, blow out the bone marrow, repeat several times until the femur and tibia are colorless, and collect after blowing Transfer the single cell suspensio