Culture media for edible mycorrhizal fungi and symbiotic seedlings and synchronous culture method by utilizing culture media

[0020] A kind of synchronous culture method that the mycorrhizal symbiosis seedling of masson pine and Poria cocos is synthetic, comprises the steps:

[0021] (1) Preparation of solid medium for mycelium expansion and propagation Weigh 20g of trehalose, 2g of yeast powder, 1g of peptone, 50ml of humus soil extract under the masson pine tree, 15g of agar, dissolve in purified water, and dilute to 1L, adjust the pH value to 5, put into test tubes or Petri dishes, and routinely sterilize.

[0022] (2) Preparation of liquid medium for cultivating mycelium for mycorrhizal symbiotic seedling inoculation Weigh 25g of trehalose, 6g of yeast powder, 2g of peptone, 50ml of humus soil extract under Pinus massoniana, dissolve in purified water, and set Make it into 1L, adjust the pH value to 5, put it into a Erlenmeyer flask, and perform routine sterilization.

[0023] (3) Poria cocos strain inoculum culture Inoculate Poria cocos hyphae on the solid medium prepared in (1) under aseptic cond

[0031] A synchronous culture method for the synthesis of mycorrhizal symbiotic seedlings of poplar and Tricholoma poplar, comprising the steps:

[0032] (1) Preparation of solid medium for mycelium expansion and propagation Weigh 24g of trehalose, 4g of yeast powder, 1.5g of peptone, 50ml of poplar humus extract, 18g of agar, dissolve in purified water, and constant volume into 1L, adjust the pH value to 5, put into test tubes or Petri dishes, and routinely sterilize.

[0033] (2) Preparation of liquid medium for cultivating mycelium for mycorrhizal symbiotic seedling inoculation Weigh 22g of trehalose, 3g of yeast powder, 1.2g of peptone, 50ml of poplar humus extract, dissolve in purified water, The volume was adjusted to 1L, the pH value was adjusted to 5, and it was filled into an Erlenmeyer flask, and routinely sterilized.

[0034] (3) Cultivation of the inoculum of Tricholoma poplaris Inoculate the mycelium of Tricholoma poplarus on the solid medium prepared in (1) under st