Methods and apparatus for improving the sensitivity of capillary zone electrophoresis
a capillary zone and electrophoresis technology, applied in the field of new methods and apparatus, can solve the problems of insufficient practical applications, insufficient concentrations of analytes found in samples, and inability to achieve many practical applications, and achieve the effect of reducing the concentration of analytes in samples to permit the use of cze separation methods
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CZE Analysis Using Preferred Concentrating Device
[0064] A column concentrating device is formulated in accordance with the principles of the present invention. The column is prepared from capillary tubes having internal diameters of 25-360 μm. The column materials are: tetraethylorthosilicate (200 μl), Ethanol (156 μl) 1.0 M nitric acid (258 μl), and chromatography particles of varying size and pore volume (e.g., 455 mg of 5 μm Ultrasphere® paticles (Beckman Coulter, Inc.).
[0065] The following column filling process is employed: Dissolve 200 μl of tetraethylorthosilicate in 156 μl of ethanol by vortexing briefly. Then add 258 μl of 1.0 M nitric acid and vortex until the solution is clear and uniform. Slowly add particles and vortex carefully to mix particles with the solution until all the particles are properly wet. Sonicate the slurry briefly to remove trapped air around the particles. Insert capillaries through a Teflon® septum into the vial containing the slurry until the end ...
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