2 results about "Corynebacterium glutamicum" patented technology

The invention discloses a Corynebacterium glutamicum recombinant strain capable of producing high-yield L-leucine, and a construction method thereof, and belongs to the field of genetic engineering. According to the present invention, the NADP-dependent branched-chain amino acid transaminase in Corynebacterium glutamicum is replaced with NAD-dependent leucine dehydrogenase (LeuDH) derived from B.sphaericus by using a genetic engineering method, such that the new efficient L-leucine synthesis pathway is constructed, the disadvantages of excessive accumulation of NADH in the Corynebacterium glutamicum L-leucine producing bacteria and insufficient supply of NADPH are solved, the L-leucine synthesis ability in the recombinant strain is enhanced, and the NADPH accumulation ability of the strainis improved; the shake fermentation experiment results show that the L-leucine accumulation of the recombinant strain achieves 16.7 g.L<-1> and is higher than the L-leucine accumulation of the starting stain of 13.2 g.L<-1>, and the maximum specific growth rate of the recombinant strain is 0.23 g.L<-1>.h<-1>, and is higher than the maximum specific growth rate of the recombinant strain of 0.18 g.L<-1>.h<-1>; and the L-leucine synthesis pathway in Corynebacterium glutamicum is successfully modified, the disadvantage of the intracellular cofactor imbalance is improved, and the new idea is provided for the breeding of high-yield L-leucine production bacteria.