Method for dynamically determining breathing oxygen consumption and hypoxia device for same

[0031] Embodiment 1 A method for dynamic determination of respiratory oxygen consumption, comprising the following steps:

[0032] 1) Put the head of the device body up, add distilled water from the vent to the water retaining strip and place it horizontally on the table. The high-sensitivity tension transducer is directly connected to the outlet, and the outlet of the anoxic bottle is connected to the side port of the high-sensitivity tension transducer;

[0033] 2) Close the three-way valve at the exhaust port, put mice into the anoxic bottle with soda lime in advance, and cap the bottle tightly;

[0034] 3) The outlet of the hypoxic bottle is connected to the connector of the transducer, and the mouse in the hypoxic bottle breathes and consumes oxygen to produce 0.5cmH 2 O negative pressure, pump the water in the oxygen consumption measurement channel to flow to the oxygen consumption capacity tank to fill the consumed oxygen capacity, so that the pressure in the entire anoxi

[0038] Example 2 A proprietary hypoxia device constructed according to the measurement method described in Example 1, including a device body 1, a hypoxia bottle, and a biological signal collection and processing system. The device body 1 is provided with an oxygen consumption capacity tank 11, an oxygen consumption Measuring channel 12, exhaust channel 13, described oxygen consumption capacity tank 11 communicates with the end of described oxygen consumption measuring channel 12; Described exhaust channel 13 communicates with described oxygen consumption measuring channel 12; The oxygen consumption measurement channel 12, the exhaust channel 13 and the oxygen consumption capacity tank 11 on the head of the main body 1 are respectively provided with corresponding vent holes 121, exhaust ports 131 and transducer connection ports 111 , the transducer connection port 111 is respectively connected with the straight port of the high-sensitivity tension transducer of the biological sign

[0047] Embodiment 3 Connect the respiratory oxygen consumption dynamic hypoxia device and the conventional oxygen consumption measuring device according to embodiment 1, adjust the length of the connecting pipeline so that the capacity of the two oxygen consumption devices is equal, add an equivalent amount of soda lime (H 3 Ca 2 NaO 4 , purchased from Shanghai Shunqiang Biotechnology Co., Ltd.), set the record acquisition parameters of RM6240 (Chengdu Instrument Factory): time constant DC, filter frequency 30Hz, sensitivity 5ml / s, sampling frequency 800Hz, scan speed 1s / div, low-pass filter 30Hz, and calibrated with a water pressure gauge. 60 mice (purchased from the Animal Center of Zhejiang University, body weight 21.0±27g, half male and half female) were weighed and marked, and two mice of the same gender and weight were randomly selected to form a pair, and one of them was placed in In the hypoxic bottle of the conventional oxygen consumption device, cap the cork tight