The invention discloses an employee training enrollment method. The method comprises the steps that basic information of each employee is collected, including post categories, management functions, interests and hobbies, career planning and credit hour requirements; enrolled employee information of set courses in a current control period is recorded, so that enrolled course information of each employee is obtained; when a new course is created, course information is acquired synchronously; and according to the basic information and the enrolled course information of the employees and the created course information, corresponding courses are automatically configured for each employee. The invention furthermore provides an application server and a computer readable storage medium. Through the employee training enrollment method, the application server and the computer readable storage medium, autonomous enrollment of employee training courses can be completed intelligently and conveniently, employee demands are met, and time and manpower investment are saved.