The invention relates to the technical field of biological 3D printing, in particular to a method for facilitating in-vitro maturation of human immature oocytes by utilizing a 3D printing technology. The method comprises the following steps of (1) properly treating naked ova and granular cells of ovary cells; (2) performing in-vitro balanced culture of ovarian granular cells; (3) putting oocytes into the ovarian granular cells, and continuing to perform culture for 2 days; (4) preparing 3D printing ink; (5) preparing 3D printing gel; and (6) printing out follicles by adopting the 3D printing technology, putting the oocytes and an in-vitro maturation culture solution into the follicles, and performing culture for one week. According to the method, the follicles with a biochemical environment similar to that of a human body try to be simulated, and then the oocytes are quickly and accurately put into the follicles for performing artificial culture by depending on the 3D printing technology, so that the in-vitro maturation of the human immature oocytes is realized; the method depends on the 3D printing technology; and the whole process is quick and convenient, and the automation degree is high, so that the technical expense of the in-vitro maturation of the oocytes can be reduced.