2 results about "High cell" patented technology

The invention relates to the technical field of cell culture and provides immune cell CAR-T culture equipment with a high cell survival rate and a culture method. The immune cell CAR-T culture equipment comprises an equipment outer frame, a culture rack and an adjusting rotating valve, wherein the equipment outer frame comprises an observation window, the culture rack comprises a culture inner chamber, the culture rack is arranged at the inner side of the equipment outer frame, a culture bin used for culturing cells is arranged at the inner side of the culture inner chamber, the observation window is arranged at the top end of a reaction tube, the reaction tube used for sensing an internal carbon dioxide concentration is movably connected to the inner side of the culture bin, and a transfer tube used for receiving nutrients is movably connected to the side, away from the reaction tube, of the culture bin. An auxiliary block drives an auxiliary clamping block to move towards the outer side through a rotating rod column, the auxiliary clamping block drives a sliding block to move towards the outer side, and a worker rapidly checks specific conditions of internal cells through the observation window, so that the survival rate of the cells can be observed at any time, and accidental death of the cells is avoided.

The invention discloses a cell tracer with low toxicity and a high cell uptake rate as well as a preparation method and application of the cell tracer. The cell tracer is a PAA and PEG coated ultra-small superparamagnetic iron oxide nanoparticle. The cell tracer is obtained by synthesizing an ultra-small superparamagnetic iron oxide nanoparticle through a thermal decomposition method and then sequentially coating PAA and PEG. The cell tracer with the low toxicity and the high cell uptake rate is obtained by coating the ultra-small superparamagnetic iron oxide nanoparticles with the PAA and thePEG, and can be used as a cell tracer applied to nuclear magnetic resonance imaging; the cell tracer can be used for efficiently marking human adipose tissue-derived stromal cells without using a transfection reagent, has no obvious toxicity to the human adipose tissue-derived stromal cells and induced pluripotent stem cells, and can be used for tracing the human umbilical cord mesenchymal stem cells transplanted into a brain of a rat.