The invention relates to an exercise bicycle service life testing machine which comprises a machine body. A table plate is arranged on the machine body. Two trapezoid grooves are formed in the table plate and are provided with a plurality of gas clamps. A mobile rack is connected with one side of the machine body. A lifting device is arranged on the mobile rack and is controlled through a handle. A direction guide plate is further arranged on the mobile rack and is provided with a guide column. A half-moon-shaped clamp is arranged on the guide column. A reducer is arranged on the half-moon clamp and is connected with a connection shaft sleeve. The connection shaft sleeve is connected with a clamping head rotating disk. A display is further arranged on the mobile rack. A to-be-tested machine is arranged on the table plate and is fastened with the machine body through two L-shaped plates. The number of the gas clamps is four. The exercise bicycle service life testing machine has the advantages of being simple in structure, simple in testing procedure, capable of adjusting in various directions, and accurate in testing result.