The invention provides an application of circular RNA as a diagnostic, therapeutic and prognostic target of oral squamous cell carcinoma, a corresponding kit, a pharmaceutical composition, and an application of the pharmaceutical composition. The CircBase ID of the circular RNA is at least one of the following IDs: hsa-circ-0093229, hsa-circ-0006208, hsa-circ-0006677 and hsa-circ-0004491. The kitfor diagnosis and/or prognosis of oral squamous cell carcinoma includes reagents for quantitative detection of the circular RNA. The circular RNA disclosed by the invention has obvious expression differences on cancer and paracancerous normal mucosal tissues of a patient with oral squamous cell carcinoma while a conventional clinical auxiliary diagnosis technology of oral squamous cell carcinoma is not perfect, so the circular RNA has the potential to be used as the diagnostic, therapeutic and prognostic target for oral squamous cell carcinoma.