The invention discloses a non-invasive nucleus implanting method for seawater pearls. The method comprises the main steps that commodity pearl cores are taken and cleaned with water firstly, and then washed using an adenosine triphosphate disodium dilution liquid; then adult healthy seawater pearl shells are taken as receptors, the upper and lower shells of receptor shells are separated by gaps using an instrument in a clean environment, the pearl cores are implanted through the feet of the pearl shells using a nucleus implanting device, then sent to the nucleus positions of the pearl shells, and the time of completion of the implanting step is controlled within one minute; the receptor shells implanted with the pearl cores are quickly placed in clean seawater for temporary rearing, and are put into a seawater culture environment within three hours and naturally cultured. The adenosine triphosphate disodium diluent liquid is prepared from 10 ml or 10 mg of commodity adenosine triphosphate disodium phosphate injection, and 50 ml of sterile water. The implanted pearl shells have no operative wound, the survival rate is increased by 20%-30% compared with using the traditional method of nucleus implanting, and the pearl rate increases by 30%-40%. Because the pearl shells do not have wound recovery process, the pearl shells grow rapidly, and the quality is excellent.