The invention discloses an in-vitro
cochlea micro-organ functional unit and a three-dimensional construction method and application thereof. Separated single mouse cochlear precursor cells are subjected to three-dimensional induction culture to form cochlear organs, the cochlear organs and spiral
neuron tissues are subjected to three-dimensional co-culture, and meanwhile, the cochlear organs are induced to be differentiated and mature into hair cells and promote growth of spiral neurons, so that a cochlear micro-organ functional unit with synaptic connection between hair cells and spiral neurons is constructed. The cochlear micro-organ functional unit provides a new thought and a good application model for auditory function reconstruction, and is used for researching a regulation and control mechanism of functional synapses formed by regenerated hair cells and spiral neurons; and a series of problems such as an
inner ear development mechanism, a
hair cell and
spiral ganglion neuron damage mechanism and
intervention measures,
hair cell regeneration regulation and control and auditory function reconstruction are better researched.