The invention relates to a wheel spoke plane difference inspection device which integrally comprises a center shaft assembly, a bearing assembly and a plane difference measurement assembly; the bearing assembly and the plane difference measurement assembly are mutually kept combined concentrically by installing the bearing assembly and the plane difference measurement assembly on the center shaft assembly concentrically, so that an inner circle plane measuring plate and a center hole guide taper as well as a height adjusting spring can carry out planar rotation and vertical sliding in a center shaft and the inner circle plane measurement plate can carry out planar rotation and vertical sliding in an excircle plane measuring plate; by arranging a standard plane, the center hole guide taper and the height adjusting spring, correct positioning can be carried out on spokes with center holes having different inner diameters, and technical conditions are provided for the plane difference measurement assembly to accurately measure the plane difference numerical value of the spoke; and by arranging the excircle plane measurement plate and the inner circle plane measuring plate, the spoke plane difference of the detected spoke can be accurately reflected. The technical scheme has remarkable functions in further perfecting the wheel detection technology and improving the detection precision on the plane difference of the spoke and the wheel product quality as well as detection efficiency.